In brief, this device aligns cells in a fluidic channel, allowing for the sequential excitation of cells by various lasers. The wavelength of the laser must be carefully selected based on the fluorescent protein being analyzed. This technique is particularly useful for obtaining a snapshot of a cell population’s distribution of cell size, morphology, and gene expression, which can be achieved by combining it with fluorescent reporters. In our lab, we have also automated our flow cytometry setup, enabling us to collect cell samples from cultivations at regular time intervals.
